How are cytology specimens processed?
Processing cytology samples involves reception of the specimen and request form, preparation of slides for microscopic examination, staining, screening and reporting the slides. All these processes are subject to quality control and quality assurance measures.
What procedures are used to obtain samples for cytological analysis?
Cytological examinations of specimens taken from different sites as a follow-up after establishing the initial diagnosis is a routine procedure. Sputum, bronchoalveolar lavage, and bronchial brushings are frequent samples that are used as follow-up for patients with a previous diagnosis of pulmonary carcinoma.
What should be done if the smear for cytologic testing Cannot be made immediately?
If it cannot be immediately delivered, add three units of heparin per mL of fluid to prevent clotting, and place in refrigerator until it can be delivered. We prefer at least 30 mL of sample; and up to 100 mL of sample if possible.
How many types of cytology are there?
What kinds of cytology are there? There are two main kinds, or branches, of cytology: exfoliative cytology and intervention cytology. Healthcare providers can use cytology tests for almost all areas of your body.
How do you perform a cytology?
Another cytology technique is to gently scrape or brush some cells from the organ or tissue being tested. The best-known cytology test that samples cells this way is the Pap test. A small spatula and/or brush is used to remove cells from the cervix (the lower part of the uterus or womb) for a Pap test.
What is a cytology sample?
Cytology is the exam of a single cell type, as often found in fluid specimens. It’s mainly used to diagnose or screen for cancer.
How accurate are cytology test?
Many studies have evaluated the accuracy of urine cytology in the detection of bladder cancer. Overall, the reported sensitivity ranges from 20% to 97.3%; specificity ranges from 74% to 99.5%.
What are the stains used in cytology?
The universal stain for cytological preparations is the Papanicolaou stain. Harris’ hematoxylin is the optimum nuclear stain and the combination of OG6 and EA50 give the subtle range of green, blue and pink hues to the cell cytoplasm.
How do you fix cytology smear?
2 The commonly used methods are air-dried and wet-fixed smears. Air- dried smears have many advantages over wet-fixed smears during routine cytology. They may be post- fixed after rehydration in saline with a variety of fixatives, such as ethanol/acetic acid, 95% ethanol or alcoholic formalin.