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EuMac mono structure

Chem3D image of EuMac_monoisothiocyanate

Recently, we have developed the monoisothiocyanate, which has the advantage that it is incapable of cross-linking macromolecules.

image of Apoptotic Cells

Image of apoptopic cells

The red emission is from the europium Quantum Dye labeled anti5-BrdU that is bound to the 5-BrdU incorporated by the apoptic cells. The blue emission is from DAPI. Both the blue and red images were obtained with a 60 x oil immersion lens. The continuous excitation was at 365 nm. Both the red and blue images were binned to 680 x 518 pixels, aligned by a program that maximizes their cross-correlation and then simulates an absorbance image by producing a psuedocolor image with a white background.


The first expected use of Quantum Dye® kits is determine the efficacy of chemotherapy drugs by sampling the tumor and surrounding tissue by fine-needle biopsies, which result in minimal trauma. These samples can be processed with Quantum Dye® reagent kits to determine the rate of programmed cell death (called apoptosis) and the rate of cell growth (cells in S-phase or cells undergoing DNA Synthesis). Presently, a Quantum Dye labeled monoclonal antibody against 5BrdU can be used for both assays. The presence of an increased number of apoptotic cells indicates response to chemotherapy. An abnormally high number of cells in S-phase indicate that the tumor is still growing and the therapy is not working. The use of a gadolinium labeled anti5BrdU should permit both apoptosis and DNA synthesis to be measured simultaneously.

image of S Phase Cells

Image of growing tissue culture cells

The red emission is from the europium Quantum Dye labeled anti5-BrdU that is bound to the 5-BrdU incorporated by the briken ends produced during the Tunnel Assay of S phase cells. The blue emission is from DAPI. Both the blue and red images were obtained with a 60 x oil immersion lens. The continuous excitation was at 365 nm. Both images were binned to 680 x 518 pixels. The cells (S) have a punctate staining pattern, which shows small islands of DNA synthesis. The cells (A) have large densely stained areas, which are the result of apoptosis induced strand breakage. The cell (?) has both types of staining.

In 1998, Newport Instruments received an STTR to research and develop additional Quantum Dye® technology. Currently, one Quantum Dye® reagent kit is in production and three more are in development.

Newport Instruments strategy for making Quantum Dye® kits a success is to rely on our experience and scientific ability to create a superior product that is economical and practical. The STTR grant has allowed Newport Instruments to develop the superior technology. Newport Instruments plans to partner with existing reagent kit producers to produce new kits based on Quantum Dye® technology. The partners already have an existing distribution network and relationship with clinical laboratories. This allows Newport Instruments to penetrate the market without having to develop these relationships from scratch. By following an OEM product development tack, Newport Instruments is positioning itself as primarily an intellectual property company. Quantum Dye® based dyes can be substituted into other existing reagent product kits to produce superior results. This OEM production leverages Newport Instruments relationships with other companies and takes advantage of larger firms marketing and distribution channels.

These advantages will permit commercial applications with the ultimate sensitivity where single molecules can be detected in solutions or bound to a solid support. This means that commercial assays can be developed to detect pathogens in foods such as fish and meat. Quantum Dye® based reagent kits can be used in clinical laboratories, dipstick based tests for bedside and doctor's office use. In addition, Quantum Dye® solutions are ideal for detecting DNA single point mutations by hybridization on bio-chips. This allows for the detection of chromosomal abnormalities (birth defects). Quantum Dye® reagent kits are no more hazardous than traditional fluorescent dye based technology and much less hazardous than the radioactive tags they replace.

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